3WN2

Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuranosidase in complex with xylohexaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-03-09
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	97.437, 97.437, 103.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.450 - 2.100
R-factor	0.16805
Rwork	0.167
R-free	0.19129
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wn1
RMSD bond length	0.007
RMSD bond angle	1.138
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	REFMAC (5.7.0029)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.450	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	29718
<I/σ(I)>	16.1	8.5
Completeness [%]	100.0	100
Redundancy	21.2	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	293	20% PEG 3350, 0.2M tri-potassium citrate, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K