3WMZ
Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuranosidase ethylmercury derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-11-03 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0031 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 97.285, 97.285, 103.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.640 - 1.900 |
R-factor | 0.18307 |
Rwork | 0.182 |
R-free | 0.19805 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.072 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.640 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 39646 | |
<I/σ(I)> | 42.9 | 13.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 28.5 | 27.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 20% PEG 3350, 0.2M tri-potassium citrate, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |