3WMW

GalE-like L-Threonine dehydrogenase from Cupriavidus necator (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	73.012, 83.487, 106.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.250
R-factor	0.22889
Rwork	0.227
R-free	0.26356
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a4v
RMSD bond length	0.008
RMSD bond angle	1.143
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.800
High resolution limit [Å]	2.250
Number of reflections	31326
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20%(w/v) PEG3350, 0.2M Calcium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K