3WMU
The structure of an anti-cancer lectin mytilec apo-form from the mussel Mytilus galloprovincialis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2013-05-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.725, 41.507, 68.831 |
| Unit cell angles | 90.00, 108.45, 90.00 |
Refinement procedure
| Resolution | 27.480 - 1.100 |
| R-factor | 0.13827 |
| Rwork | 0.137 |
| R-free | 0.16277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.298 | |
| Number of reflections | 120340 | |
| Completeness [%] | 96.0 | 89.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Sodiumu acetate, HEPES, Glycerol, PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
