3WME
Crystal structure of an inward-facing eukaryotic ABC multidrug transporter
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-06-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9929, 1.0000, 1.0053, 1.0060, 1.0091 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 180.312, 180.312, 152.332 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.817 - 2.751 |
| R-factor | 0.2125 |
| Rwork | 0.209 |
| R-free | 0.27510 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.088 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.059 | 0.038 | 0.371 |
| Number of reflections | 24596 | ||
| <I/σ(I)> | 16.1 | ||
| Completeness [%] | 99.3 | 87.8 | 100 |
| Redundancy | 7.9 | 7.3 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 15% (w/v) PEG 2000 MME, 100mM magnesium nitrate, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
