3WLU
Crystal Structure of human galectin-9 NCRD with Selenolactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97889 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.732, 68.167, 224.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.400 |
| R-factor | 0.17332 |
| Rwork | 0.172 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.736 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.086 | 0.885 |
| Number of reflections | 109285 | |
| <I/σ(I)> | 19.3 | 3.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 289 | PEG 6000, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, temperature 289K |
