3WLP

Crystal Structure Analysis of Plant Exohydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9615
Spacegroup name	P 43 21 2
Unit cell lengths	99.939, 99.939, 182.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.410 - 1.570
R-factor	0.16327
Rwork	0.162
R-free	0.18296
Starting model (for MR)	1ieq
RMSD bond length	0.016
RMSD bond angle	1.761
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.020	1.610
High resolution limit [Å]	1.570	1.570
Rmerge		0.510
Number of reflections	118580
Completeness [%]	97.0	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	75mM HEPES-NaOH pH7.0, 1.2% PEG 400, 1.7M ammonium sulphate , VAPOR DIFFUSION, HANGING DROP, temperature 277K