3WLA
Crystal Structure of sOPH Native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.340, 75.742, 75.980 |
| Unit cell angles | 74.04, 88.67, 74.01 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wl5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.840 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.510 |
| Number of reflections | 92945 | |
| <I/σ(I)> | 26.2 | 2.9 |
| Completeness [%] | 97.8 | 96.5 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2M Li2SO4, 0.1 M Bis-Tris pH 6.5, 3% v/v Polyethylene Glycol 500, 6% v/v Polyethylene Glycol 600, 25% w/v Polyethylene Glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
