3WKY
Crystal structure of hemolymph type prophenoloxidase (proPOb) from crustacean
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 156.706, 156.706, 283.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.806 - 1.801 |
| R-factor | 0.1757 |
| Rwork | 0.175 |
| R-free | 0.19550 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 | 1.860 |
| High resolution limit [Å] | 1.800 | 2.270 | 1.800 |
| Rmerge | 0.098 | 0.477 | |
| Number of reflections | 239565 | ||
| <I/σ(I)> | 15.3 | 2.46 | |
| Completeness [%] | 99.7 | 100 | 98.4 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1M Sodium malonate, 0.1 M HEPES pH 7.0, 0.5% Jeffamine ED-2001 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
