3WKR
Crystal structure of the SepCysS-SepCysE complex from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 106.880, 106.880, 495.253 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.300 - 2.803 |
| R-factor | 0.2297 |
| Rwork | 0.224 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.534 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.706 | |
| Number of reflections | 74017 | 6424 |
| <I/σ(I)> | 9.28 | 1.48 |
| Completeness [%] | 95.0 | 94.6 |
| Redundancy | 4.11 | 3.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | Ammonium citrate, PEG3350, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
