3WKM
The periplasmic PDZ tandem fragment of the RseP homologue from Aquifex aeolicus in complex with the Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.243, 86.042, 92.542 |
| Unit cell angles | 100.84, 96.92, 101.32 |
Refinement procedure
| Resolution | 47.440 - 2.200 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nsn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.440 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 74923 | |
| Completeness [%] | 98.2 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 16%(W/V) POLYETHYLENE GLYCOL 8000, 200MM SODIUM MALONATE, 0.5%(W/) N-OCTYL BETA-D-GLUCOPYRANOSIDE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
