3WKM
The periplasmic PDZ tandem fragment of the RseP homologue from Aquifex aeolicus in complex with the Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | P 1 |
Unit cell lengths | 51.243, 86.042, 92.542 |
Unit cell angles | 100.84, 96.92, 101.32 |
Refinement procedure
Resolution | 47.440 - 2.200 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nsn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.174 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 74923 | |
Completeness [%] | 98.2 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 16%(W/V) POLYETHYLENE GLYCOL 8000, 200MM SODIUM MALONATE, 0.5%(W/) N-OCTYL BETA-D-GLUCOPYRANOSIDE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |