3WKL

The periplasmic PDZ tandem fragment of the RseP homologue from Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-21
Detector	ADSC QUANTUM 270
Spacegroup name	P 65 2 2
Unit cell lengths	78.861, 78.861, 211.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.440 - 2.800
R-factor	0.257
Rwork	0.255
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zpl 2zpm
RMSD bond length	0.007
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	10257
Completeness [%]	99.8	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		1.5M AMMONIUM SULFATE, 0.1M SODIUM ACCETATE, PH 4.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K