3WJW
Wild-type orotidine 5'-monophosphate decarboxylase from M. thermoautotrophicus complexed with 6-methyl-UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.025, 103.573, 73.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.590 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.18590 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3wjy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.780 | 1.630 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.069 | 0.302 |
| Number of reflections | 30174 | |
| <I/σ(I)> | 29.5 | 6.4 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | Sodium citrate, pH 6, VAPOR DIFFUSION, temperature 293K |
