3WJM
Crystal structure of Bombyx mori Sp2/Sp3 heterohexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.056, 205.020, 119.713 |
| Unit cell angles | 90.00, 103.00, 90.00 |
Refinement procedure
| Resolution | 48.370 - 2.800 |
| R-factor | 0.17523 |
| Rwork | 0.172 |
| R-free | 0.23739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 97193 | |
| Completeness [%] | 96.5 | 88.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG3350, MgCl2, Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
