3WJ9
Crystal structure of the eukaryotic initiation factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.443, 93.443, 269.387 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.721 - 2.506 |
| R-factor | 0.2403 |
| Rwork | 0.238 |
| R-free | 0.25840 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 47788 | |
| <I/σ(I)> | 49.8 | 5.3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 22.1 | 22.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.3 | 293 | 6% PEG 8000, 0.1M magnesium acetate, 0.1M sodium acetate, pH 4.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
