3WI8
Crystal structure of horse heart myoglobin reconstituted with manganese porphycene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.828, 28.657, 63.744 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.200 |
| R-factor | 0.18904 |
| Rwork | 0.186 |
| R-free | 0.25319 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 6299 | |
| <I/σ(I)> | 24.9 | 3.2 |
| Completeness [%] | 97.8 | 95.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 3.2M ammonium sulfate, 0.1M imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
