3WI4
Crystal structure of wild-type PorB from Neisseria meningitidis serogroup B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.291, 148.291, 104.045 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.320 |
| R-factor | 0.2579 |
| Rwork | 0.254 |
| R-free | 0.29352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.840 | 3.370 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 6628 | |
| Completeness [%] | 92.8 | 76.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M MES pH 6.5-7.0, 50mM CsCl, 35% (v/v) Jeffamine M-600, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
