3WI0

P453H/I471T mutant of PB2 middle domain from influenza virus A/Puerto Rico/8/34(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-08-05
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	H 3 2
Unit cell lengths	107.818, 107.818, 136.615
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.975 - 1.998
R-factor	0.1868
Rwork	0.182
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vqz
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.980	2.090
High resolution limit [Å]	1.998	1.998
Number of reflections	20909
Completeness [%]	99.7	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	1.2M NaCl, 2.5% (v/v) ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K