3WHK
Crystal structure of PAN-Rpt5C chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.040, 85.760, 105.260 |
| Unit cell angles | 90.04, 90.03, 89.89 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h4m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.471 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.081 | 0.381 |
| Number of reflections | 73505 | |
| <I/σ(I)> | 17.2 | 6.9 |
| Completeness [%] | 96.2 | 97.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 8% PEG3350, 0.1M Bis-Tris (pH6.0), 0.1M magnesium formate, 3mM CHAPSO, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
