3WH6
Crystal structure of GH1 beta-glucosidase Td2F2 glucose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.087, 68.454, 95.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.230 - 1.600 |
| R-factor | 0.15885 |
| Rwork | 0.157 |
| R-free | 0.19037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ug6 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.276 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 59793 | |
| <I/σ(I)> | 33 | 3 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.2M K/Na tartrate, 0.1M Na-CHES, 0.2M Li2SO4, 0.5M D-glucose, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
