3WGJ
STAPHYLOCOCCUS AUREUS FTSZ T7 chimera mutant, T7Bs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.955, 43.862, 86.488 |
| Unit cell angles | 96.91, 102.34, 109.38 |
Refinement procedure
| Resolution | 40.493 - 2.179 |
| R-factor | 0.1879 |
| Rwork | 0.185 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3voa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.189 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.179 | 2.180 |
| Number of reflections | 29191 | |
| Completeness [%] | 96.0 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M tri-Sodium citrate, 20%(w/v) PEG4000, 20%(w/v) Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
