3WGG
Crystal structure of RSP in complex with alpha-NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.816, 74.137, 54.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.900 - 2.100 |
| R-factor | 0.18198 |
| Rwork | 0.179 |
| R-free | 0.23124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 3WG9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.818 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.072 | 0.456 |
| Number of reflections | 33930 | |
| <I/σ(I)> | 20.7 | 2.5 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 20% w/v PEG 3350, 0.4M ammonium sulfate, 0.1M Bis-Tris, plus 5mM alpha-NAD+, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
