3WGE

Crystal structure of ERp46 Trx2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-03
Detector	RAYONIX MX225HE
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	32.580, 39.060, 87.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.476 - 0.950
R-factor	0.1228
Rwork	0.122
R-free	0.13480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.370
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.570	1.000
High resolution limit [Å]	0.950	0.950
Number of reflections	70957
Completeness [%]	99.9	100
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1M HEPES, 2.2M Ammonium sulfate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K