3WG4
Crystal structure of Agrocybe cylindracea galectin mutant (N46A) with blood type A antigen tetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 102.824, 102.824, 74.578 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.680 - 1.600 |
| R-factor | 0.14911 |
| Rwork | 0.148 |
| R-free | 0.17485 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ww7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.258 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.070 | 0.055 | 0.427 |
| Number of reflections | 59122 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 11.3 | 10.8 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 30% PEG 1500, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
