3WFT
Crystal structure of horse heart myoglobin reconstituted with cobalt(II) tetradehydrocorrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.975, 28.987, 63.214 |
| Unit cell angles | 90.00, 106.14, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.12887 |
| Rwork | 0.126 |
| R-free | 0.18294 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 3.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 28032 | |
| <I/σ(I)> | 26.9 | |
| Completeness [%] | 92.8 | 90.1 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1M Tris-HCl, 3.4M ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
