3WFN
Crystal Structure of Nav1.6 IQ motif in complex with apo-CaM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.830, 50.752, 150.666 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 48.095 - 1.950 |
| R-factor | 0.2011 |
| Rwork | 0.200 |
| R-free | 0.23670 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.952 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 83450 | |
| Completeness [%] | 99.5 | 94.9 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M Bis-Tris Propane pH 6.5, 55% Tacsimate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
