3WEM
Sugar beet alpha-glucosidase with acarviosyl-maltotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 97.443, 139.377, 149.365 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.363 - 2.591 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w37 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.363 | 2.750 | |
| High resolution limit [Å] | 2.590 | 7.670 | 2.590 |
| Rmerge | 0.107 | 0.024 | 0.664 |
| Rmeas | 0.026 | 0.731 | |
| Number of reflections | 31809 | 1321 | 4956 |
| <I/σ(I)> | 16.87 | 48.68 | 3.14 |
| Completeness [%] | 99.4 | 98.1 | 97.3 |
| Redundancy | 5.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 50mM ammonium sulfate, 50mM sodium acetate buffer, 16% PEG monomethylether 2000, 15.8mM acarviosyl-maltotetraose, pH 4.5, vapor diffusion, hanging drop, temperature 298K |
