3WE5
Crystal structure of ubiquitin conjugating enzyme E2 UbcA1 from Agrocybe aegerita
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.930, 34.760, 128.100 |
| Unit cell angles | 90.00, 108.88, 90.00 |
Refinement procedure
| Resolution | 34.641 - 1.700 |
| R-factor | 0.2053 |
| Rwork | 0.202 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fo3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.125 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.204 |
| Number of reflections | 37008 | |
| <I/σ(I)> | 9.7 | 3.4 |
| Completeness [%] | 94.1 | 72.2 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 3350, 0.2M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
