3WDP
Structural analysis of a beta-glucosidase mutant derived from a hyperthermophilic tetrameric structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.150, 161.145, 183.824 |
| Unit cell angles | 90.00, 108.38, 90.00 |
Refinement procedure
| Resolution | 47.050 - 1.700 |
| R-factor | 0.21285 |
| Rwork | 0.212 |
| R-free | 0.23281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3apg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.050 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.086 |
| Number of reflections | 367711 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1M HEPES-NaOH pH7.6, 1.2M Ammomium di-hydrogen Phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
