3WDO
Structure of E. coli YajR transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0063,1.0089,0.9791 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 128.725, 128.725, 165.149 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.168 - 3.150 |
| R-factor | 0.2723 |
| Rwork | 0.271 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 14541 | |
| Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 289 | 30% PEG300, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, temperature 289K |
| 1 | VAPOR DIFFUSION | 5 | 289 | 30% PEG300, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, temperature 289K |
