3WDH
Crystal structure of Pullulanase from Anoxybacillus sp. LM18-11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-04 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.895, 65.780, 90.494 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.750 |
R-factor | 0.165 |
Rwork | 0.165 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.051 | 0.490 |
Number of reflections | 83162 | |
<I/σ(I)> | 30.8 | 4.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 295 | 0.1M HEPES, pH 7.5, 7%(w/v) Polyethylene Glycol 8000, 10%(v/v) Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 295K |