3WD8
TypeIII polyketide synthases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.724, 135.912, 107.623 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 19.987 - 2.463 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cgk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.987 | 2.510 |
| High resolution limit [Å] | 2.463 | 2.470 |
| Number of reflections | 52852 | |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 50mM Tris-HCl, 18% PEG3350, 4%(v/v) 1-propanol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
