3WCN
Crystal structure of the depentamerized mutant of selenocysteine synthase SelA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 144.137, 144.137, 273.406 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.321 - 3.350 |
| R-factor | 0.1928 |
| Rwork | 0.191 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w1i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.102 | 0.712 |
| Number of reflections | 21096 | |
| <I/σ(I)> | 17.2 | 2.75 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100mM trisodium citrate-HCl, 580-590mM trisodium citrate, 100mM L-serine, 100mM Na2S2O3, pH 6, vapor diffusion, sitting drop, temperature 293K |
