3WBM
Crystal Structure of protein-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9642 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.890, 69.760, 48.130 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 43.804 - 2.000 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.672 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.407 |
| Number of reflections | 37589 | |
| <I/σ(I)> | 11 | 3.1 |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% (v/v) PEG 5000 MME, 100mM Bis-Tris, 200mM sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
