3WBK
crystal structure analysis of eukaryotic translation initiation factor 5B and 1A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.939, 120.942, 132.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| R-factor | 0.2613 |
| Rwork | 0.257 |
| R-free | 0.31730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wbi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.306 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
| Rmerge | 0.071 | 0.033 | 0.492 |
| Number of reflections | 24875 | ||
| <I/σ(I)> | 16.2 | ||
| Completeness [%] | 98.5 | 94.4 | 92.9 |
| Redundancy | 9.4 | 9.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 100mM Tris-HCl pH 8.2, 12.5%(w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
