3WBK

crystal structure analysis of eukaryotic translation initiation factor 5B and 1A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-28
Detector	RAYONIX MX225HE
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	101.939, 120.942, 132.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.300
R-factor	0.2613
Rwork	0.257
R-free	0.31730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wbi
RMSD bond length	0.008
RMSD bond angle	1.306
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.071	0.033	0.492
Number of reflections	24875
<I/σ(I)>	16.2
Completeness [%]	98.5	94.4	92.9
Redundancy	9.4	9.7	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	100mM Tris-HCl pH 8.2, 12.5%(w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K