3WAP
Crystal structure of Atg13 LIR-fused human LC3C_8-125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-01-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.730, 61.730, 95.881 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.460 - 3.100 |
| R-factor | 0.21045 |
| Rwork | 0.207 |
| R-free | 0.27321 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.445 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.460 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.069 | 0.785 |
| Number of reflections | 4045 | |
| <I/σ(I)> | 15.8 | 2.8 |
| Completeness [%] | 98.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.1M Sodium acetate trihydrate, 8% PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
