3WAI
Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (AfAglB-L, O29867_ARCFU) from Archaeoglobus fulgidus as a MBP fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.239, 52.930, 78.418 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 29.793 - 1.897 |
| R-factor | 0.1657 |
| Rwork | 0.164 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1peb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (Version 1.8_1069 (phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.897 | 1.900 |
| Rmerge | 0.071 | 0.586 |
| Number of reflections | 58383 | |
| <I/σ(I)> | 25 | 2.5 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 293 | 0.1M CAPSO, 33% PEG 3350, pH 9.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
