3WAF
X-ray structure of apo-TtFbpA, a ferric ion-binding protein from thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.150, 99.050, 132.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.17697 |
| Rwork | 0.175 |
| R-free | 0.20959 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xvy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.290 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 47714 | |
| Completeness [%] | 87.8 | |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 0.1M SODIUM CITRATE TRIBASIC DEHYDRATE (PH5.2), 20% (W/V) 2-PROPANOL, 20% (W/V) PEG 4000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
