3WA7
Crystal structure of selenomethionine-labeled tannase from Lactobacillus plantarum in the orthorhombic crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9788 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.730, 77.820, 199.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.910 - 1.700 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.19300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.159 |
| Number of reflections | 42594 | |
| <I/σ(I)> | 20.6 | 8.5 |
| Completeness [%] | 88.7 | 60.6 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 297 | PEG8000, sodium acetate, ammonium sulfate , pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
