3WA5

Crystal Structure of type VI peptidoglycan muramidase effector Tse3 in complex with its cognate immunity protein Tsi3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2012-06-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	109.970, 109.970, 84.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 1.900
R-factor	0.1623
Rwork	0.160
R-free	0.20210
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX ((1.7.3_928))
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.180	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.105	0.335
Number of reflections	41634
<I/σ(I)>	28.9	11.8
Completeness [%]	100.0	100
Redundancy	28.4	28.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% Jaffamine ED-2001, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K