3WA1
Crystal structure of BinB: A receptor binding component of the binary toxin from Lysinibacillus sphaericus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 95.210, 95.210, 154.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.640 - 1.750 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.21000 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.197 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 42536 | |
| <I/σ(I)> | 4.4 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 295 | 0.1M TRIS-HCL PH8.0, 0.2M LITHIUM SULFATE, 12% PEG 4000, VAPOR DIFFUSION, TEMPERATURE 295K |
