3W9Z
Crystal structure of DusC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 93.252, 93.252, 115.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.097 - 2.100 |
| R-factor | 0.2213 |
| Rwork | 0.221 |
| R-free | 0.23520 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.168 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 30439 | |
| <I/σ(I)> | 71.7 | 5.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 15.3 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.9 | 293 | 0.1M Tris pH7.9, 0.2M Na Acetate, 12% PEG 4000, VAPOR DIFFUSION, temperature 293K |
| 2 | VAPOR DIFFUSION | 8 | 293 | 0.1M Imidazole pH8.0, 15% Isopropanol, 20% Glycerol , VAPOR DIFFUSION, temperature 293K |
