3W9Y
Crystal structure of the human DLG1 guanylate kinase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979077 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 65.535, 65.535, 180.563 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.000 - 2.200 |
R-factor | 0.215 |
Rwork | 0.209 |
R-free | 0.26500 |
Structure solution method | MR-SAD |
Starting model (for MR) | 1jxo |
RMSD bond length | 0.008 |
RMSD bond angle | 1.001 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 12192 | |
<I/σ(I)> | 25.3 | 2.9 |
Completeness [%] | 98.2 | 97.6 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium thiocyanate, 22% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |