3W9E
Structure of Human Monoclonal Antibody E317 Fab Complex with HSV-2 gD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-10-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.029, 91.087, 141.537 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.780 - 2.300 |
| R-factor | 0.20613 |
| Rwork | 0.203 |
| R-free | 0.25544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l2g 3w9d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.354 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.061 |
| Number of reflections | 37832 |
| <I/σ(I)> | 26.2 |
| Completeness [%] | 98.5 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.2 | 295 | 30%(v/v) PEG 550MME, 0.1M Na HEPES, pH 8.2, VAPOR DIFFUSION, temperature 295K |
