3W9D
Structure of Human Monoclonal Antibody E317 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-20 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.781, 90.722, 128.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.320 |
| R-factor | 0.19396 |
| Rwork | 0.190 |
| R-free | 0.25929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.320 |
| Rmerge | 0.055 |
| Number of reflections | 40644 |
| <I/σ(I)> | 29.3 |
| Completeness [%] | 96.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 298 | 25%(w/v) PEG 4000, 10mM calcium chloride dihydrate, 100mM sodium citrate tribasic dihydrate, pH 5.8, VAPOR DIFFUSION, temperature 298K |
