3W9A
Crystal structure of the catalytic domain of the glycoside hydrolase family 131 protein from Coprinopsis cinerea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.200, 68.421, 69.168 |
| Unit cell angles | 89.99, 72.87, 85.81 |
Refinement procedure
| Resolution | 30.040 - 1.990 |
| R-factor | 0.19089 |
| Rwork | 0.188 |
| R-free | 0.23587 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 2.070 |
| High resolution limit [Å] | 1.986 | 2.000 |
| Rmerge | 0.105 | 0.289 |
| Number of reflections | 66894 | |
| Completeness [%] | 94.7 | 88.2 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 7% PEG4000, 100mM ammonium sulfate, 100mM sodium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
