3W8V
Crystal Structure Analysis of the synthetic GCN4 coiled coil peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.168, 34.162, 95.067 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.380 - 2.100 |
| R-factor | 0.2007 |
| Rwork | 0.192 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ij2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.093 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 23.000 | 23.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.670 | 2.100 |
| Rmerge | 0.106 | 0.051 | 0.416 |
| Number of reflections | 6278 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.1 | 98.9 | 90.7 |
| Redundancy | 10.2 | 9.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 50% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
