3W8P
MamM-CTD D249A&H28A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.947 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.560, 87.526, 64.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.580 - 1.800 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.976 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.870 | 1.800 |
| Rmerge | 0.087 | 0.062 | 0.501 |
| Number of reflections | 19801 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 99.4 | 94.4 | 100 |
| Redundancy | 10.4 | 8.8 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.1M BIS-TRIS PH=6.2, 26% PEG 3350, 0.2M AmSO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
