3W8N
Open form structure of CMP kinase in complex with CMP from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-10 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 31 |
| Unit cell lengths | 49.212, 49.212, 98.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.620 - 2.200 |
| Rwork | 0.199 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.049 | 0.032 | 0.262 |
| Number of reflections | 26811 | ||
| <I/σ(I)> | 23.3 | ||
| Completeness [%] | 99.5 | 99.4 | 99.2 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10mM CMP, 10mM MgCl2, 0.6M sodium succinate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
