3W8K
Crystal structure of class C beta-lactamase Mox-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.617, 59.378, 62.685 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.16485 |
| Rwork | 0.164 |
| R-free | 0.18913 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zkj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.321 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.273 | 1.398 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 72411 | |
| Completeness [%] | 99.0 | 86.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% PEG8000, 0.2M zinc acetate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
